[A sensitive analytical method for six aflatoxins in rainbow trout muscle and liver].
نویسندگان
چکیده
A highly sensitive analysis method for six aflatoxins (aflatoxin B₁, B₂, G₁, G₂, M₁ and aflatoxicol) in rainbow trout muscle and liver was developed. Aflatoxins (AFs) were extracted with acetonitrile-water (9 : 1), purified on an immunoaffinity column, and subjected to HPLC with fluorescence detection after post-column photochemical derivatization. The recoveries of AFs at 0.05 μg/kg spiking levels were 71.4-82.4% in muscle and 80.1-93.0% in liver, and the repeatability relative standard deviations (RSDr) were 0.87-4.6% in muscle and 2.0-6.2% in liver. Limits of quantitation (LOQs) and limits of detection(LODs)of AFs were estimated to be 0.004-0.029 μg/kg, and 0.002-0.012 μg/kg, respectively.
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عنوان ژورنال:
- Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
دوره 52 3 شماره
صفحات -
تاریخ انتشار 2011